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. 2019 Sep 19;18(3):770–778. doi: 10.1111/pbi.13244

Figure 2.

Figure 2

Adenine base editing in rice by ABE‐P2S and ABE‐P5S. a, Diagram of the ABE‐P2, ABE‐P2S, ABE‐P5 and ABE‐P5S base editors. b, Schematic view of the sgRNA8 target site in SPX‐MFS2. c, Sequence chromatogram of one homozygous line, Line 38, at the SPX‐MFS2 target site edited by ABE‐P2S. d, Sequence chromatogram of Line 8 at the OsSPL14 target site edited by ABE‐P2S. The sgRNA9 target site in OsSPL14 and OsSPL17 is shown. e, Sequence chromatogram of Line 24 at the OsSPL18 target site edited by ABE‐P2S. The sgRNA10 target site in OsSPL16 and OsSPL18 is shown. f, Schematic view of the sgRNA11 target site in OsSPL13. g, Sequence chromatogram of Line 3 at the OsSPL13 target site edited by ABE‐P5. h, Sequence chromatograms of Line 23 and Line 27 at the OsSPL13 target site edited by ABE‐P5S. The PAM sequence and miRNA binding site are highlighted in red and blue, respectively (b, d, e, f). Sequence chromatograms of lines with no mutation at the corresponding target sites are used as controls (c, d, e, g, h). Arrows point to the positions with an edited base (c, d, e, g, h).