Table 1.
sgRNA | Target gene | Base editor | Rice variety | Number of lines genotyped | Number of base edited lines | Editing efficiency (%) |
---|---|---|---|---|---|---|
sgRNA1 | OsSPL14 | ABE‐P1 | Nipponbare | 48 | 18 | 37.5 |
ABE‐P1S | Nipponbare | 17 | 12 | 70.6 | ||
sgRNA2 | SLR1 | ABE‐P1 | Nipponbare | 40 | 7 | 17.5 |
ABE‐P1S | Nipponbare | 20 | 7 | 35 | ||
sgRNA3 | OsSERK2 | ABE‐P1 | Nipponbare | 39 | 26 | 66.7 |
ABE‐P1S | Nipponbare | 27 | 21 | 77.8 | ||
sgRNA4 | Tms9‐1 | ABE‐P1 | Kitaake | 22 | 1 | 4.5 |
ABE‐P1S | Kitaake | 36 | 4 | 11.1 | ||
sgRNA5 | OsNRT1.1B | ABE‐P1 | Kitaake | 37 | 27 | 72.9 |
ABE‐P1S | Kitaake | 54 | 52 | 96.3 | ||
sgRNA6 | OsACC1 | ABE‐P1 | Kitaake | 36 | 25 | 69.4 |
ABE‐P1S | Kitaake | 42 | 31 | 73.8 | ||
sgRNA7 | OsDEP1 | ABE‐P1 | Kitaake | 50 | 40 | 80 |
ABE‐P1S | Kitaake | 35 | 25 | 71.4 |