Table 2.

Summary of base editing efficiencies at different sites for ABE‐P2, ABE‐P2S, ABE‐P5 and ABE‐P5S

sgRNA	Target gene	Base editor	Rice variety	Number of lines genotyped	Number of base edited lines*	Editing efficiency (%)
sgRNA8	SPX‐MSF2	ABE‐P2	Nipponbare	41	4	9.8
		ABE‐P2S	Nipponbare	47	7	15.9
sgRNA9	OsSPL14	ABE‐P2	Nipponbare	28	11 (11)	39.3
	OsSPL17	ABE‐P2	Nipponbare	28	13 (11)	46.4
	OsSPL14	ABE‐P2S	Nipponbare	36	20 (15)	55.6
	OsSPL17	ABE‐P2S	Nipponbare	36	22 (15)	61.1
sgRNA10	OsSPL16	ABE‐P2S	Nipponbare	33	10 (5)	30.3
	OsSPL18	ABE‐P2S	Nipponbare	33	13 (5)	39.4
sgRNA11	OsSPL13	ABE‐P5	Nipponbare	46	1	2.2
		ABE‐P5S	Nipponbare	33	2	6.1
sgRNA12	SNB	ABE‐P5	Nipponbare	22	2	9.1
		ABE‐P5S	Nipponbare	62	21	33.9

*The number in () indicates the number of lines that were edited at both target sites simultaneously.