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. 2019 Sep 17;18(3):791–804. doi: 10.1111/pbi.13247

Figure 6.

Figure 6

TaCYP81D5 confers salinity tolerance by promoting Zat12‐mediated ROS signalling pathway, thereby enhancing ROS scavenging. (a) The H2O2 and (b) MDA contents in WT, TaOE‐null, TaOE1 and TaOE2 wheat lines. (c) ROS levels of WT, TaOE‐null, TaOE1 and TaOE2 wheat lines by carboxy‐H2 DCFDA staining. Bar: 0.2 cm. (d) The abundance of TaCAT , TaAPX , TaNOX and TaAOX transcript in roots of WT, TaOE‐null, TaOE1 and TaOE2 wheat lines. TaEF1‐α (M90077) (Paolacci et al., 2009) was chosen as the endogenous control. Relative transcript abundance of every gene in WT was calculated by giving the value 1. (e) CAT and (f) APX activities of WT, TaOE‐null, TaOE1 and TaOE2 wheat lines. (g) The transcript abundance of TaZat12 and TaZFP36 in roots of SR3 and JN177. Relative transcript abundance of every gene in JN177 was calculated by giving the value 1. (h) The transcript abundance of TaZat12 and TaZFP36 in roots of WT, TaOE‐null, TaOE1 and TaOE2 wheat lines. Relative transcript abundance of every gene in WT was calculated by giving the value 1. (i) Y1H assay showing that TaZat12 could bind the A(G/C)T repeats element in TaAPX promoter. TaAPX‐P: the ~500‐bp promoter fragment of TaAPX ; TaAPX‐mP : a fragment deleting the A(G/C)T repeats element; SD/‐Leu, SD medium without Leu; SD/‐Leu/AbA400, SD medium without Leu supplemented with AbA at the concentration of 400 ng/mL. Transformed yeast cells were dotted at 10−1 dilutions on the selective medium. (j) Transient expression assay showing that TaZat12 could activate the expression of TaAPX , and the A(G/C)T repeats element is essential for this activation. The black column represents the relative LUC/REN ratio of the reporter in the present of empty effector (pBI221 empty vector) without TaZat12; and the grey column represents the relative LUC/REN ratio of the reporter in the present of effector containing TaZat12. Data are presented as mean ± SE of at least three biological replicates. Columns marked with one asterisk indicate significant differences (P < 0.05) using Student's t‐test, and double asterisks indicate P < 0.01.