Table 1.
Genetic analysis of CRISPR/Cas9‐induced mutations in BnaMAX1s and their transmission to the T1 and T2 generations.
| T0 plants line | T1 plants | T2 plants | ||||
|---|---|---|---|---|---|---|
| No. of tested plants | Line | Chr. A03 | Chr. C03 | No. of homozygous plants | Plants without T‐DNA | |
|
S1‐5 a1 + T a2 WT c1 − CTT c2 WT |
6 | S1‐5‐1 | + T/WT (He) | − CTT (Ho) | 10/24 | None |
| S1‐5‐4 | + T (Ho) | − CTT (Ho) | 8/8 | None | ||
| S1‐5‐6 | + T (Ho) | − CTT (Ho) | 12/12 | S1‐5‐6‐8 | ||
|
S1‐8 a1 + T a2 WT c1 + T c2 WT |
60 | S1‐8‐8 | + T (Ho) | + T (Ho) | 8/8 | None |
| S1‐8‐12 | + T (Ho) | + T (Ho) | 10/10 | None | ||
| S1‐8‐23 | WT | WT | 0/23 | S1‐8‐23‐3/7/10/14/17/18 | ||
| S1‐8‐36 | + T/WT (He) | + T (Ho) | 0/2 | None | ||
| S1‐8‐39 | + T (Ho) | + T (Ho) | 11/11 | S1‐8‐39‐1/4/6/10 | ||
|
S1‐11 a1 − T a2 + T c1 + A c2 + T |
47 | S1‐11‐1 | − T (Ho) | + T (Ho) | 20/20 | S1‐11‐4/7/12/19 |
| S1‐11‐7 | − T/+ T (Bi‐a) | + A (Ho) | 10/22 | All | ||
| S1‐11‐13 | − T/+ T (Bi‐a) | + T (Ho) | 9/22 | All | ||
| S1‐11‐16 | − T (Ho) | + T (Ho) | 22/22 | All | ||
|
S2‐5 a1 + T a2 GC − T c1 + GT c2 + G |
14 | S2‐5‐2 | GC − T (Ho) | + G (Ho) | 16/16 | S2‐5‐2‐6/10/15 |
| S2‐5‐6 | + T/GC − T (Bi‐a) | + GT/+ G (Bi‐a) | 4/16 | S2‐5‐6‐3/5 | ||
| S2‐5‐14 | + T (Ho) | + GT/+ G (Bi‐a) | 6/16 | S2‐5‐14‐2/8 | ||
He, heterozygous; Ho, homozygous; Bi‐a, bio‐allelic. ‘−’ and ‘+’ indicate the deletion and insertion in the sgRNAs target sites, respectively.
Bold indicates the numbers of T0 plants.