FIGURE 3.

The simulations mimicking the comparison of depolarization-induced translocation of PKCγ and DGKγ molecules in the mutant and wild-type models of PCs. These results show the membrane-to-cytosol (M/C) ratio of PKCγ and DGKγ molecules in response to a brief 1-min pulse, which leads to the rapid generation of DAG in the membrane compartment. Here, the strength of stimulation is controlled by setting the pulse parameter “S₁” at 20. The generation of the second messenger, in turn, stimulates the translocation of both PKCγ and DGKγ from cytosol to membrane. Here, the solid line represents the non-stimulation and the dashed line represents the stimulation condition (green dashed line, mutant; red dashed line, wild-type PCs). (A) The translocation characteristics of the PKCγ molecule in both mutant and wild-type models. These results suggest that for identical strength and duration of stimulation, the cytosol-to-membrane migration kinetics of PKCγ molecule are much faster in mutant models compared to wild-types. Compared to wild-types, the membrane residence time of PKCγ molecule is shorter in mutant models, i.e., 7.2 s for mutant and 18 s for wild-type models. (B) The translocation characteristics of the DGKγ molecule in both mutant and wild-type models. These results show that the membrane residence time of the DGKγ molecule is shorter in mutant models compared to models representing wild-type PCs.