FIGURE 4.

The simulations mimicking the effect of stimulation strength on the comparative translocation kinetics of PKCγ isoform in the mutant and wild-type models of PCs. Here, the strength of stimulation is controlled by setting the pulse parameter “S₁” at different levels. The parameter S₁ is set at arbitrary values of 20, 40, and 60 (inset). The duration of pulse for all these three cases is 1 min. Here, the solid line represents the non-stimulation and the dashed line represents the stimulation condition (green dashed line, mutant; red dashed line, wild-type PCs). (A) Translocation characteristics of PKCγ in the mutant model. Here, results show that maximum levels of M/C ratio of PKCγ increases with increase in the strength of parameter “S₁”; however, the residence time of mutant PKCγ first decreases and then increases with pulse strength (S₁ = 20, leads to maximum M/C levels of 5 and τ_PKCγmutant = 7.2 s; S₁ = 40, leads to maximum M/C levels of 11 and τ_PKCγmutant = 6.9 s; S₁ = 60, leads to maximum M/C levels of 20 and τ_PKCγmutant = 7.4 s). (B) Translocation characteristics of PKCγ in the wild-type models. Here, results show that maximum levels of the M/C ratio of PKCγ increases with increase in the strength of parameter “S₁,” and the residence time of wild-type PKCγ decreases with pulse strength (S₁ = 20, leads to maximum M/C levels of 10 and τ_PKCγ = 18 s; S₁ = 40, leads to maximum M/C levels of 64 and τ_PKCγ = 9 s; S₁ = 60, leads to maximum M/C levels of 100 and τ_PKCγ = 8.6 s).