FIGURE 5.

The simulations mimicking the effect of feed forward rate constant of DAG phosphorylation by DGKγ_P on the comparative translocation kinetics of PKCγ molecule in the mutant and wild-type models of PCs. Parameter k₆ represent this rate constant in mutant models, whereas in wild-types, it is represented by k₈. Here, the strength of stimulation is controlled by setting the pulse parameter “S₁” at 20. The duration of pulse is set for 1 min. Here, the solid line represents the non-stimulation and the dashed line represents the stimulation condition (green dashed line, mutant; red dashed line, wild-type PCs). (A) Translocation characteristics of PKCγ in the mutant model. Here, results show that increasing the parameter k₆ in the mutant model reduces the membrane residence time of PKCγ (at baseline, k₆, τ_{PKCγ mutant} = 7.2 s; and 25–100 times increase in k₆ leads to τ_PKCγmutant = 6.2 s). (B) Translocation characteristics of PKCγ in the wild-type models. Here, results show that increasing the parameter k₈ in the wild-type model reduces the membrane residence time of PKCγ (at baseline, k₈, τ_PKCγ = 18 s; and 25–100 times increase in k₈ leads to τ_PKCγ = 16 s).