FIGURE 7.

The simulations mimicking the effect of blocking the rate constant of DGKγ phosphorylation to DGKγ_P on the comparative translocation kinetics of PKCγ molecule in the mutant and wild-type models of PCs. Parameter k₄ represents this rate constant in mutant models, whereas in wild-types, it is represented by k₆. Here, the strength of stimulation is controlled by setting the pulse parameter “S₁” at 20. The duration of pulse is set for 1 min; the solid line represents the non-stimulation and the dashed line represents the stimulation condition (green dashed line, mutant; red dashed line, wild-type PCs). (A) Translocation characteristics of PKCγ in the mutant model. Here, results show that blocking the parameter k₄ in the mutant model increases the membrane residence time of PKCγ (baseline, k₄, τ_{PKCγ mutant} = 7.2 s; 80% blocking of k₄ leads to τ_{PKCγ mutant} = 18 s; 90% blocking of k₄ leads to τ_{PKCγ mutant} = 23 s). (B) Translocation characteristics of PKCγ in the wild-type models. Here, results show that blocking the parameter k₈ in the wild-type model reduces the membrane residence time of PKCγ (baseline, k₆, τ_PKCγ = 18 s; 80% blocking leads to τ_PKCγ = 12 s; 90% blocking leads to τ_PKCγ = 14 s).