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. 2020 Feb 6;11(2):98. doi: 10.1038/s41419-020-2288-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Confocal images of cortical slices stained for PECAM-1 (green) and laminin (red) to visualise blood vessels at 7 days in vitro, scale bar 500 μm. b–d Different stages of vascular sprouting can be visualised in cortical brain slices including; tip cell formation (*) endothelial tip extension (framed area in b which is expanded in c) new sprouting (arrow heads) and bridging (arrow) (scale bar 50 μm) (d). e Diagram summarising the different stages of sprouting angiogenesis that can be observed in cortical organotypic slice cultures.