Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 6;10:2008. doi: 10.1038/s41598-020-58495-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Cloning of MID1. (A) Diagram of mid1 mutant. The Ds insertion is pointed out by a triangle. The position of primers used in (C,D) are displayed by arrows. (B) The phenotype of 4-week-old wild-type (WT), mid1, and genetically complemented lines MID1Pro:MID1-3 × Flag and 35SPro:MID1-GFP. Bar = 1 cm. (C) Genotyping of plants in (B) using the primers indicated in (A). (D) RT-PCR detection of MID1 cDNA in plants (B) to confirm the successful complementation. The primers F1 and R1 were used to RT-PCR analysis of MID1.