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. 2020 Feb 6;10:2008. doi: 10.1038/s41598-020-58495-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The subcellular localization and expression pattern of MID1. (A) Schematic structure of MID1. MTS, mitochondrial transient signal. (B) Subcellular localization of MID1-GFP in Arabidopsis protoplasts. Bar = 10 μm. (C) Subcellular localization of MID1-GFP in the stomata of 35SPro:MID1-GFP transgenic seedling. Bar = 5 μm. (D) Immunodetection of MID1-GFP localization. α-VDAC and α-TIC40 are mitochondrion and chloroplast-specific antibodies, respectively. (E–I) The expression pattern of MID1-GUS reporter. (E) seedling, arrow indicates shoot apex, (F) flower, (G) root meristem, (H) lateral root primordium, (I) unfertilized ovule and (J) ovule with heart embryo, arrow indicate the embryo. (E,F) Bars = 1 mm, (G–J), Bars = 50 μm. (K) Relative expression levels of MID1 mRNA in diverse tissues measured by qRT-PCR. The mean data are derived from triple independent biological replicates prepared from three parallel samples, and finally normalized with ACTIN2.