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. 2020 Feb 6;10:2008. doi: 10.1038/s41598-020-58495-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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MID1 is associated with other trans-factors involved in nad2 intron 1 splicing. (A) Y2H analysis of interaction relationship between trans-factors involved in nad2 intron 1 splicing. The upper panel displays the yeast that can grow on the SD/-Trp-Leu (SD/-T-L) dropout media. The lower panel displays the yeast growing on the SD/-Ade-His-Trp-Leu (SD/-A-H-T-L) dropout media, which indicates the physical interaction of two trans-factors. 10 mM 3-amino-1, 2, 4-triazole (3-AT) is added in the media to inhibit the autoactivation of MTSF1(43–997aa) BD + AD and BD + ODB1(47–176aa) + AD. (B) Co-IP analysis of trans-factors association in Arabidopsis protoplast. About 70 μg total proteins were loaded in the input lanes and one sixth volume of GFP beads were loaded in the IP lanes. Protein mark is on right side of immunoblot. (C) Luciferase complementation assay of interaction between MID1, MTSF1 and itself. The pseudocolor bar indicates the relative luminescence intensity in the image.