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. 2020 Feb 6;10:2008. doi: 10.1038/s41598-020-58495-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Functional analysis of each domain of MID1. (A) Schematic diagram of MID1 variants. (B) The phenotypes of mid1-1 complemented by MID1 variants. Bar = 1 cm. (C) RT-PCR analysis of splicing efficiency of nad2 intron 1 in mid1-1 complemented by MID1 variants. ACTIN2 is used as loading control.