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. 2020 Jan 31;10:52. doi: 10.3389/fonc.2020.00052

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Copyright © 2020 Guo, Kuang, Wu, Wen, Zhou, Liao, Song, Xu, Wang, Jing, Li, Hu, Ling, Wang and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Inhibiting HK2 activates AMPK and subsequently inhibits mTORC1 activity. (A) Western blot showing the expression of phospho-AMPK in H226 si-HK2- and H520 si-HK2-transfected cells compared with that in siNC-transfected cells or cells treated with different doses of lonidamine. (B) Immunoblot showing the expression of phosphor-S6K1 and phosphor-4EBP1 in H226 siHK2- and H520 si-HK2-transfected cells compared with that in H226 siNC- and H520 siNC-transfected cells, respectively. (C,D) Seahorse metabolic analysis of the extracellular acidification rate (ECAR) in H226 si-NC- and siHK226-transfected cells. (E,F) Seahorse metabolic analysis of ECAR in H520 si-NC- and siHK520-transfected cells. (G,H) Seahorse metabolic analysis of oxygen consumption rates (OCRs) in H226 si-NC- and siHK226-transfected cells. (I,J) Seahorse metabolic analysis of OCRsin H520 si-NC- and siHK520-transfected cells. *p < 0.05.