Table 4.
Signal ratios of the four products (PA–PD), TIM blank dialysate (T bl), non-spiked dialysate sample (TxS), dialysate samples spiked at 3.0 mg/ml (TxL, end concentration 0.25 mg/ml), dialysate samples spiked at 15 mg/ml (TxH, end concentration 1.25 mg/ml), dialysate sample signal ratios corrected for blank dialysate (Corr stands for corrected), the outcome of the division of product signal ratios by TxS Corr (12.5 is the end concentration ratio when 100% would go to the dialysate; values < 12.5 indicate that the TIM dialysate contains more than product equivalent; values > 12.5 indicate that the TIM dialysate contains less than product equivalent). Finally, two quality parameters, which can be used to assess whether an obtained result is analytically acceptable: the additional signals for spike low and spike high samples and the outcome of the division (value close to 5 indicates linear signal in the concentration range) and the product signal ratios divided by the additional signals for spike high (should be 3.33 when there is no matrix effect)
| Sample | m/z 244 | m/z 260 | m/z 286 | m/z 357 | Sum/division of sums |
|---|---|---|---|---|---|
| PA | 783.88 | 534.29 | 34.31 | 708.25 | 2060.73 |
| PB | 33.29 | 2243.26 | 85.06 | 699.29 | 3060.90 |
| PC | 82.50 | 116.99 | 47.06 | 128.09 | 374.65 |
| PD | 21.90 | 4903.73 | 282.45 | 995.51 | 6203.59 |
| T bl | 1.46 | 25.57 | 4.59 | 0.94 | 32.56 |
| TAS | 15.11 | 108.68 | 26.95 | 80.74 | 231.49 |
| TAL | 41.52 | 133.09 | 26.03 | 118.56 | 319.19 |
| TAH | 189.84 | 258.89 | 59.30 | 287.85 | 795.88 |
| TAS Corr (TAS–T bl) | 13.64 | 83.11 | 22.36 | 79.81 | 198.92 |
| TAL Corr (TAL–T bl) | 40.05 | 107.52 | 21.44 | 117.62 | 286.63 |
| TAH Corr (TAH–T bl) | 188.38 | 233.31 | 54.71 | 286.91 | 763.32 |
| PA/TAS Corr | 57.46 | 6.43 | 1.53 | 8.87 | 10.36 |
| A TAL Corr–TAS Corr | 26.41 | 24.41 | − 0.93 | 37.81 | 87.70 |
| B TAH Corr–TAS Corr | 174.73 | 150.20 | 32.35 | 207.10 | 564.39 |
| B/A | 6.62 | 6.15 | − 34.92 | 5.48 | 6.44 |
| PA/B | 4.49 | 3.56 | 1.06 | 3.42 | 3.65 |
| TBS | 5.59 | 219.72 | 11.48 | 37.60 | 274.38 |
| TBL | 7.65 | 338.42 | 15.41 | 68.69 | 430.17 |
| TBH | 12.76 | 912.95 | 42.41 | 217.31 | 1185.44 |
| TBS Corr (TBS–T bl) | 4.12 | 194.15 | 6.89 | 36.67 | 241.82 |
| TBL Corr (TBL–T bl) | 6.19 | 312.84 | 10.82 | 67.76 | 397.61 |
| TBH Corr (TBH–T bl) | 11.30 | 887.38 | 37.82 | 216.38 | 1152.87 |
| PB/TBS Corr | 8.07 | 11.55 | 12.35 | 19.07 | 12.66 |
| A TBL Corr–TBS Corr | 2.07 | 118.70 | 3.93 | 31.09 | 155.79 |
| B TBH Corr–TBS Corr | 7.18 | 693.23 | 30.93 | 179.71 | 911.05 |
| B/A | 3.47 | 5.84 | 7.86 | 5.78 | 5.85 |
| PB/B | 4.64 | 3.24 | 2.75 | 3.89 | 3.36 |
| TCS | 6.26 | 39.07 | 25.21 | 31.08 | 101.61 |
| TCL | 9.03 | 92.93 | 57.44 | 86.43 | 245.82 |
| TCH | 29.49 | 60.75 | 38.96 | 58.56 | 187.75 |
| TCS Corr (TCS–T bl) | 4.80 | 13.50 | 20.62 | 30.14 | 69.05 |
| TCL Corr (TCL–T bl) | 7.57 | 67.35 | 52.85 | 85.49 | 213.26 |
| TCH Corr (TCH–T bl) | 28.02 | 35.17 | 34.37 | 57.62 | 155.18 |
| PC/TCS Corr | 17.20 | 8.67 | 2.28 | 4.25 | 5.43 |
| A TCL Corr–TCS Corr | 2.77 | 53.86 | 32.23 | 55.35 | 144.21 |
| B TCH Corr–TCS Corr | 23.23 | 21.68 | 13.75 | 27.48 | 86.13 |
| B/A | 8.39 | 0.40 | 0.43 | 0.50 | 0.60 |
| PC/B | 3.55 | 5.40 | 3.42 | 4.66 | 4.35 |
| TDS | 13.94 | 476.60 | 23.77 | 89.13 | 603.43 |
| TDL | 10.53 | 792.01 | 41.80 | 133.70 | 978.04 |
| TDH | 20.55 | 1945.92 | 131.05 | 323.37 | 2420.88 |
| TDS Corr (TDS–T bl) | 12.48 | 451.02 | 19.18 | 88.19 | 570.87 |
| TDL Corr (TDL–T bl) | 9.07 | 766.43 | 37.21 | 132.76 | 945.48 |
| TDH Corr (TDH–T bl) | 19.09 | 1920.34 | 126.46 | 322.43 | 2388.32 |
| PD/TDS Corr | 1.76 | 10.87 | 14.73 | 11.29 | 10.87 |
| A TDL Corr–TDS Corr | − 3.41 | 315.41 | 18.03 | 44.57 | 374.61 |
| B TDH Corr–TDS Corr | 6.61 | 1469.32 | 107.28 | 234.24 | 1817.45 |
| B/A | − 1.94 | 4.66 | 5.95 | 5.26 | 4.85 |
| PD/B | 3.31 | 3.34 | 2.63 | 4.25 | 3.41 |