Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 6;11:741. doi: 10.1038/s41467-020-14537-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — Chromophore (HBI) and its neighboring residues in the rsEGFP2 laser_off structure determined by SFX without pump-laser activation are shown. a The initial laser-off model consists of a mixture of the off-state conformer (light gray carbon trace) and the residual on-state conformer (yellow carbon trace) with occupancies of 90% and 10%, respectively. 2F_obs^laser_off–F_calc^laser_off (blue) and F_obs^laser_off–F_calc^laser_off (green/red) maps at 1.6 Å resolution are displayed at 1σ and ±3σ, respectively. A positive F_obs^laser_off –F_calc^laser_off peak between the trans (gray) and the cis (yellow) chromophores suggests the presence of an additional conformer. b Result of ensemble refinements against the laser_off dataset starting for the off-state model (chromophore 100% trans1). A third chromophore conformation and a third rotamer of His149 are revealed. (c) The final laser-off model features triple conformations of His149 and of the chromophore, i.e. His149-off and trans1 (light gray), His149-on and cis (yellow) and the additional His149-supp and trans2 (dark gray) conformations, at 65%, 10% and 25% occupancy, respectively. 2F_obs^laser_off–F_calc^laser_off (blue) and F_obs^laser_off–F_calc^laser_off (green/red) maps calculated from the laser-off dataset at 1.6 Å resolution are displayed at 1σ and ±3σ, respectively.