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. 2020 Feb 6;10:2048. doi: 10.1038/s41598-020-58817-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Nucleotide substitution rates per site between ptDNAs of two Ectocarpus species. (A) Nucleotide substitution frequency (counts per each window) between ptDNAs of two species of E. siliculosus plotted on the full ptDNA sequence. The window size is 100 bp and the maximum value is 100. There are several peaks of high variability especially in the IRs and the V-region. Four major peaks: (a) to (d) in the V-region are magnified in panel B. (B) Each peak corresponds to the flanking region of a syntenic block described in Fig. 3 (violet boxes). (C,D) Biparental inheritance of ptDNA was confirmed by mapping sequence reads from a hybrid onto region (b) of the parental ptDNA sequences. (E) Nucleotide substitution plot between male and female Sc. promiscuus individuals from the same population. The window size is 100 bp and the maximum value is 50. There were few substitutions between the two individuals.