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. 2019 Dec 16;32:69–84. doi: 10.1016/j.molmet.2019.11.014

Figure 1.

Figure 1

ARCAgRP/NPY→ PVH projections increase in fasted WT mice.(A, B) Representative photomicrographs of the ARC and PVH coronal sections of mice in each experimental group, respectively, subjected to immunofluorescence against NPY (Pseudo-colored to green), (F, G) AgRP (red), and (K, L) POMC (pseudo-colored to magenta). Insets in each image show high magnification of areas marked in low magnification images. Arrows point at positive cells. Scale bars: 100 μm (low magnification) and 10 μm (high magnification). Cell nuclei labeled with Hoechst (blue). (C–E) Bar graphs displaying the quantitative analysis of the mean fluorescence intensity, fluorescent area and integrated density of the NPY- (n = 4 per group), (H–J) AgRP- (n = 4 per group), and (M–O) POMC-positive signal (n = 4 per group) in the PVH of each experimental group. Data represent the mean ± SEM and were compared by unpaired t-test with Welch's correction. *p < 0.05, **p < 0.01 and ***p < 0.001 vs. fed condition.