Figure 2.

ARC^NPY→PVH projections increase in fasted NPY-GFP mice.(A, B) Representative photomicrographs showing the ARC and PVH coronal sections of NPY-GFP mice in each experimental group, respectively. Insets in each image show high magnification of areas marked in low magnification images. Arrows point at GFP-positive cells. Scale bars: 100 μm (low magnification) and 10 μm (high magnification). (C–E) Bar graphs displaying the quantitative analysis of the mean fluorescence intensity, fluorescent area and integrated density of the GFP-positive signal in the PVH of each experimental group (n = 5 per group). (F) Representative photomicrographs of the ARC coronal sections of NPY-GFP mice in each experimental group subjected to chromogenic immunostaining against c-Fos (Pseudo-colored to magenta). Insets in each image show high magnification of areas marked in low magnification images. Arrows point at dual-labeled cells. Scale bars: 100 μm (low magnification) and 10 μm (high magnification). Cell nuclei labeled with Hoechst (blue). (G) Bar graph displaying the percentage of GFP+ cells positive for c-Fos in the ARC in each experimental condition (n = 4 and n = 6 for fed and fasted group, respectively). Data represent the mean ± SEM and were compared by unpaired t-test with Welch's correction. **p < 0.01 and ***p < 0.001 vs. fed condition.