Figure 6.

Fasting-induced increase in ARC^AgRP/NPY→ PVH projections is impaired in GHSR-deficient mice.(A, B) Representative photomicrographs of the ARC and PVH coronal sections, respectively, of WT and GHSR-deficient mice in each experimental group, subjected to immunofluorescence against AgRP (red). Insets in each image depict high magnification images of the areas marked in low magnification images. Arrows point at AgRP-positive cells. Scale bars: 100 μm (low magnification) and 10 μm (high magnification). Cell nuclei labeled with Hoechst (blue). (C) Bar graph displaying the quantitative analysis of the mean fluorescence intensity of the AgRP-positive signal in the ARC of each experimental group (n = 4–5). (D–F) Bar graphs displaying the quantitative analysis of the mean fluorescence intensity, fluorescent area and integrated density of the AgRP-positive signal in the PVH of each experimental group (n = 4–5). (G, H) Bar graphs displaying the quantitative analysis of the number of c-Fos-positive cells in the ARC and PVH, respectively, of each experimental group (n = 7–11). Data represent the mean ± SEM and were compared by two-way ANOVA. *p < 0.05, **p < 0.01 and ***p < 0.001 vs. different condition, same genotype; ^##p < 0.01 and ^###p < 0.001 vs. same condition, different genotype.