Figure 7.

Fasting-induced increase in ARC^NPY→ PVH projections is impaired in NPY-GFP/GHSR-deficient mice.(A, B) Representative photomicrographs of the ARC and PVH coronal sections, respectively, of NPY-GFP/WT and NPY-GFP/GHSR-deficient mice in each experimental group. Insets in each image depict high magnification images of the areas marked in low magnification images. Arrows point at GFP-positive cells. Scale bars: 100 μm (low magnification) and 10 μm (high magnification). Cell nuclei labeled with Hoechst (blue). (C) Bar graph displaying the quantitative analysis of the mean fluorescence intensity of the GFP-positive signal in the ARC of each experimental group (n = 5–6). (D–F) Bar graphs displaying the quantitative analysis of the mean fluorescence intensity, fluorescent area and integrated density of the GFP-positive signal in the PVH of each experimental group (n = 5–6). Data represent the mean ± SEM and were compared by two-way ANOVA. **p < 0.01 and ***p < 0.001 vs. different condition, same genotype; ^#p < 0.05 vs. same condition, different genotype.