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. 2020 Feb 6;10:1991. doi: 10.1038/s41598-020-58522-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Development of macro colony formation in A. baumannii17978. (A) Stereo macroscopic images of single colonies of A. baumannii upon the expression of individual GGDEF/EAL domain proteins from the plasmid pMMB67EH. Growth was done on congo red agar plates supplemented with 1 mM IPTG and 100 μg/ml carbenicillin. The diguanylate cyclases A1S_2506 and A1S_3296 mediated expression of reddish matrix component at 30 °C and 37 °C after 48 hours.