Figure 2.

S100A12 expression in peripheral monocytes from periodontitis patients and healthy controls. (A) Monocyte counts (x10⁹/l) in participants with periodontitis (n = 5) and controls (n = 5). (B) Frequency of classical, intermediate, and non-classical monocytes in participants with periodontitis (n = 5) and controls (n = 5). (C) Representative contour plots of S100A12⁺ classical (red), intermediate (blue), and non-classical (orange) monocytes in periodontitis and control. Proportion of S100A12⁺ cells in each subset is included in the plot. (D) Percentage of S100A12⁺ monocytes in periodontitis patients (n = 5) and controls (n = 5). Monocytes were identified based on the expression of CD14, CD16, and HLA-DR. (E) Percentage of S100A12⁺ cells in classical, intermediate, and non-classical monocytes from periodontitis patients (n = 5) and controls (n = 5). (F) Secretion of S100A12 in monocytes from periodontitis patients (n = 5) and controls (n = 5) after 24 h in culture. (G) S100A12 levels in plasma from periodontitis patients (n = 5) and controls (n = 5). Data are presented as mean ± SEM. Differences were calculated with Mann–Whitney test, *p < 0.05.