Figure 3.

Secretion of S100A12 in a 3D oral tissue culture with and without monocytes after inflammatory stimulation. (A) Time-dependent secretion of S100A12 in cultures with (n = 4) and without healthy human monocytes (n = 3) after 3, 5, and 7 days in culture. (B) S100A12 secretion on day 7 after monocyte implantation after repeated stimulation with LPS and IFN-γ in cultures with (n = 6) and without monocytes (n = 4). (C) S100A12 secretion by monocytes (n = 3) after 24 h stimulation with conditioned medium (CM) from cultures without monocytes (n = 3). (D) Immunohistochemical staining of S100A12 or isotype control in an oral tissue culture with monocytes. Arrows indicate S100A12⁺ cells. Data are presented as mean ± SEM. Differences were calculated with Wilcoxon test or Friedman test with Dunn's post-hoc test, *p < 0.05, **p < 0.001.