Figure 3.

LUCRC was required for colorectal cancer cell proliferation, migration, and invasion in vitro and tumorigenesis in vivo. (A) HCT116 cells were transfected with control siRNA (siCTL) or siRNA specifically against (siLUCRC) for 3 days before FACS analysis. Percentage of apoptotic cells (debris) were also shown on the top. (B) HCT116 cells were infected with control shRNA (shCTL) or two independent shRNA specifically targeting LUCRC (shLUCRC) for duration as indicated before cell proliferation assay (± s.e.m., ***P < 0.001). (C) HCT116 cells were infected with shCTL or two independent shLUCRC for 10 days before colony formation assay (± s.e.m., **P < 0.01, ***P < 0.001). (D) HCT116 cells as described in (B, C) were subjected to RNA extraction and RT-qPCR analysis to examined the expression of LUCRC (± s.e.m., ***P < 0.001). (E) HCT116 cells were transfected with siCTL or siLUCRC for 2 days and then re-seeded at full confluence and maintained for duration as indicated before wound-healing assay. (F) Quantification of wound closure as shown in (E) (± s.e.m., **P < 0.01, ***P < 0.001). (G) HCT116 cells were transfected with siCTL or siLUCRC for 2 days and then re-seeded at full confluence and maintained for 1 day before transwell assay. (H) Quantification of cells as shown in (G) (± s.e.m., **P < 0.01). (I) ShCTL or shLUCRC-infected HCT116 cells were injected subcutaneously into female BALB/C nude mice for xenograft experiments. (J) Tumor weight as shown in (I) (± s.e.m., **P < 0.01). (K) Colorectal tumor tissues and the corresponding adjacent normal tissues were collected from a group of colorectal cancer patients (n = 14) and subjected to RNA extraction and RT-qPCR analysis to examine the expression of LUCRC (± s.e.m., ***P < 0.001).