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. 2020 Feb 6;10:1973. doi: 10.1038/s41598-020-58584-5

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Images of larvae (Hemicentrotus pulcherrimus) are shown at 10 days post fertilization. Larvae were fed algae and can be seen with full stomachs. Wild-type control animals also have deep red pigment cells throughout the body of the larva. Animals in which guide RNAs were injected for PKS, FMO3, or GCM develop, swim, and feed normally, but they have no pigmentation. Scale bar = 100 microns.