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. 2020 Feb 6;10:1973. doi: 10.1038/s41598-020-58584-5

Figure 5.

Figure 5

Tube feet are shown under polarized and DIC microscopy. Tube feet from control animals show the detailed patterning of the skeletal elements characteristic of this tissue as well as the intense red pigment of the pigment cells throughout the tip and base of each tube foot. The tube feet from animals lacking PKS also contain intricate skeletal elements in their tube feet, but have no detectable pigment in the tip, base, or elsewhere in the tube feet. This phenotype is indistinguishable from the GCM-null animals (data not shown). Tube feet of the FMO3-null animals appear indistinguishable from the wild-type tube feet. They have detailed skeletal elements, and pigment cells, seen in the tips of the tube feet by polarized light, and in the base of the tube feet seen by DIC. Scale bar = 50 microns.