Fig. 2. Anchor screens of BCL2L1 and MCL1 recover known and novel interactions.

a Average log2-fold changes for guides in Meljuso cells for control and BCL2L1 knockout lines. Points are colored by density. Pearson correlation coefficient is indicated. b Residuals for guides from the BCL2L1 anchor screen in Meljuso. Blue and red lines correspond to lethal and buffering guides respectively. Density of all guides is indicated by the gray distribution. c Top 13 hits ranked by absolute average Z-score across all BCL2L1 screens. Color scale of Z-scores is shown to the right. A violin plot representing the distribution of Z-scores is adjacent to the color scale, along with two dotted lines representing the cutoffs for gene hits shown. The color scale is floored at −5 and ceilinged at 5. d Top 13 hits ranked by absolute average Z-score across all MCL1 screens, as in c. e Comparison of average Z-scores for all MCL1 and BCL2L1 perturbations screened with the Brunello library. Genes with an absolute average Z-score greater than 5 in either condition are labeled.