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. 2020 Feb 6;3:58. doi: 10.1038/s42003-020-0780-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — a Overall view of protein and RNA interactions with P site tRNA in IC2B. The color code is the same as in Figs. 1 and 2. Modified rRNA nucleotides are in red and unmodified nucleotides are in black. uS13 is shown in dark green, uS19 in cyan green and uS9 in light green. h44 is in black. Putative magnesium ions are in green spheres. For clarity, aIF2 subunits have not been drawn. b Closeup of P site interactions. c View of IC2B P site tRNA and surrounding universal proteins uS9, uS13, and uS19. d View of P site tRNA from a eukaryotic PIC obtained in the absence of eIF1 shown in the same orientation as view c (PDB: 6FYX¹⁷, eIF5 was not drawn for clarity).