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. 2020 Feb 6;3:58. doi: 10.1038/s42003-020-0780-0

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Surface representation of successive P. abyssi translation initiation complexes. IC0-P_REMOTE and IC1-P_IN are from ref. ¹². tRNA accommodation, h44 adjustments and aIF2 release from h44 are associated with the release of aIF1. After start codon recognition, aIF1 is released and the initiator tRNA is fully accommodated. Release of aIF1 would cause both Pi release from aIF2γ and h44 adjustments leading to irreversible aIF2 release (see discussion).