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. 2020 Feb 6;10:1984. doi: 10.1038/s41598-019-56877-y

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Sox2-induced FN fibrillogenesis is responsible for Schwann cell organization and persistent migration. (a) Representative confocal images of SOX2 (cyan), Actin fibres (red) and FN1 (green) immunostainings of SC^wt, SC^Sox2/Cl2 and SC^Sox2/FnKO. Arrow heads show the organization of the actin fibres in the SC^Sox2/Cl2. Scale bar 100 μm. (b,c) Correlation of FN1 fibre angles with the orientation of actin fibres in SC^wt and SC^Sox2/Cl2 (N = 3) (d,e) Quantification of FN1 anisotropy of SC^wt and SC^Sox2/Cl2 and actin fibres of SC^wt, SC^Sox2/Cl2 and SC^Sox2/FnKO (N = 3, n ≥ 12 areas) (f) Representative time-lapse imaging from Supplementary Movies 5–7 from SC^wt, SC^Sox2/Cl2 and SC^Sox2/FnKO respectively, cultured in KSRM (FN-free). Color lines show single-cell tracks of the Schwann cells during 42 hours. Scale bar, 100 μm. (g,h) Quantification of the distance and persistence of SC^wt, SC^Sox2/Cl2 and SC^Sox2/FnKO migration in KSR medium, quantification was performed considering only 100 frames per cell (n = 10 cells). Graphs show the mean ± s.e.m. **p < 0.005, ***p < 0.0005.