Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Feb 6;10:1984. doi: 10.1038/s41598-019-56877-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Sox2 and FNEIIIA are up-regulated and co-expressed in Schwann cells after sciatic nerve transection. (a) Representative confocal images of FN1 (green) and SOX2 (red) immunostainings of rat sciatic nerves in control conditions or after complete transection, at day 4, 7 and 10 post surgery. Nuclei were counterstained with DAPI (blue). Scale bar, 50 μm. (b,c) Real-time RT-qPCR measurements of Sox2 and the Fn1 containing the spliced domain EIIIA from control and transected nerves at day 4, 7 and 10 post surgery. Ankrd27 and Rictor were used as reference genes, graphs show the mean value ± s.e.m. t-test comparing cut nerves with the controls in each time point, *p < 0.05 **p < 0.005, ***p < 0.0005. (N = 3) (d) In situ hybridization using a probe against Fn1EIIIA (purple) and SOX2 DAB staining (brown) in control and transected nerves at day 4, 7 and 10 post surgery. Insets in the bottom panels show the co-expression of Fn1EIIIA with a Sox2-positive Schwann cell at day 7 and 10 post surgery. Scale bar, 50 μm.