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. 2020 Feb 6;10:2062. doi: 10.1038/s41598-020-59038-8

Figure 4.

Figure 4

Bone regeneration capacity in the mandibular bone defect of minipigs evaluated by histological analysis and immunohistochemical staining at 12 weeks after implantation. (a) Haematoxylin and Eosin (H&E)-stained sections of the empty and seeded scaffolds at 5x and 10× magnifications at 12 weeks with scale bar = 500 µm and 100 µm respectively. H&E stain showed in-vivo new bone tissue formation with osteocytes. (b) Immunohistochemical staining for Osteocalcin in the empty and pADSC-seeded scaffolds at 5x and 10× magnifications (scale bar = 500 and 100 µm respectively). (c) Quantification of Osteocalcin staining. The area of bone labelling positive for OC was recorded in % of total bone area. Significantly higher amount of osteocalcin deposition was found in the test group (p < 0.001). Data presented as means ± SD (n = 3).