. 2020 Feb 6;10:2060. doi: 10.1038/s41598-020-59029-9

Table 2.

pH, dissolved oxygen (DO), electrical conductivity (EC) and biomass concentration (B) during petroleum degradation assays with Bacillus thuringiensis, Bacillus pumilus and Rhodococcus hoagii at different cultivation periods.

	Cultivation time (h)	pH		DO (mg L⁻¹)		EC (mS cm⁻¹)		B (g L⁻¹)
	Cultivation time (h)	CT	IT	CT	IT	CT	IT	CT	IT
Bacillus thuringiensis	0	6.58 ± 0.20 ^aA	6.74 ± 0.14^bA	1.49 ± 0.11^bA	1.24 ± 0.13 ^aA	1678.67 ± 57.83^bcA	5.65 ± 0.08^bB	0.00 ± 0.00^aB	1.43 ± 0.03^bA
	24	6.71 ± 0.13^aB	7.06 ± 0.01 ^aA	1.26 ± 0.03^cA	0.72 ± 0.06^bB	1737.00 ± 89.94^bA	5.76 ± 0.17^bB	0.00 ± 0.00^aB	6.20 ± 0.42 ^aA
	48	6.85 ± 0.19 ^aA	7.04 ± 0.05 ^aA	1.80 ± 0.04 ^aA	0.79 ± 0.06^bB	1843.33 ± 37.90^abA	6.02 ± 0.05^aB	0.00 ± 0.00^aB	7.58 ± 0.54 ^aA
	72	6.90 ± 0.09 ^aA	6.87 ± 0.06 ^aA	1.74 ± 0.09 ^aA	0.61 ± 0.16^bB	1864.67 ± 52.79^abA	6.12 ± 0.13^aB	0.00 ± 0.00^aB	4.61 ± 1.19 ^aA
	96	6.79 ± 0.10 ^aA	6.89 ± 0.12 ^aA	1.42 ± 0.04^bcA	0.67 ± 0.06^bB	1940.67 ± 43.82 ^aA	6.82 ± 0.38^aB	0.00 ± 0.00^aB	4.03 ± 0.57 ^aA
Bacillus pumilus	0	6.68 ± 0.21^bA	6.73 ± 0.09 ^aA	1.37 ± 0.03 ^aA	0.60 ± 0.26^aB	1618.00 ± 209.36 ^aA	5.48 ± 0.15^bB	0.01 ± 0.01^aB	1.03 ± 0.74^bA
	24	6.52 ± 0.20^bA	6.59 ± 0.15 ^aA	0.98 ± 0.05^bA	0.49 ± 0.29^aB	1651.00 ± 104.92 ^aA	5.48 ± 0.17^bB	0.00 ± 0.00^aB	2.82 ± 1.62 ^aA
	48	6.56 ± 0.16^bA	6.82 ± 0.25 ^aA	0.84 ± 0.14^bA	0.44 ± 0.10^aB	1788.33 ± 150.32 ^aA	6.19 ± 0.10^aB	0.00 ± 0.00^aB	3.29 ± 0.62 ^aA
	72	7.43 ± 0.23 ^aA	6.65 ± 0.25^aB	0.95 ± 0.07^bA	0.82 ±± 0.15 ^aA	1567.67 ± 91.98 ^aA	6.21 ± 0.50^aB	0.00 ± 0.00^aB	2.98 ± 0.24 ^aA
	96	6.47 ± 0.22^bA	6.60 ± 0.13 ^aA	0.96 ± 0.08^bA	0.44 ± 0.05^aB	1598.67 ± 243.67 ^aA	6.82 ± 0.09^aB	0.00 ± 0.00^aB	3.83 ± 0.18 ^aA
Rhodococcus hoagii	0	6.92 ± 0.26 ^aA	6.46 ± 0.38 ^aA	2.52 ± 0.23 ^aA	2.19 ± 0.15^bA	1796.67 ± 63.96 ^aA	5.90 ± 0.54^aB	0.02 ± 0.01^aB	1.70 ± 0.64 ^aA
	24	6.59 ± 0.15 ^aA	6.62 ± 0.38 ^aA	2.01 ± 0.20 ^aA	2.02 ± 0.53^bA	1797.67 ± 60.75 ^aA	5.77 ± 0.57^aB	0.05 ± 0.15^aB	2.50 ± 0.33 ^aA
	48	6.59 ± 0.57 ^aA	6.74 ± 0.31 ^aA	2.10 ± 0.15 ^aA	1.94 ± 0.20^bA	1759.33 ± 10.97 ^aA	6.11 ± 0.08^aB	0.00 ± 0.00^aB	3.10 ± 0.88 ^aA
	72	6.60 ± 0.14 ^aA	6.25 ± 0.19 ^aA	2.48 ± 0.20^aB	3.96 ± 0.21 ^aA	1860.33 ± 42.00 ^aA	7.37 ± 2.07^aB	0.00 ± 0.00^aB	2.93 ± 0.88 ^aA
	96	6.41 ± 0.02 ^aA	5.86 ± 0.15^bB	2.40 ± 0.28^aB	3.17 ± 0.41 ^aA	1755.67 ± 50.46 ^aA	6.13 ± 0.12^aB	0.08 ± 0.02^aB	2.53 ± 0.54 ^aA

Averages followed by the same lowercase letters in the same column do not differ statistically by Tukey’s test (p < 0.05). Averages followed by the same uppercase letters in the same row do not differ statistically by Tukey’s test (P < 0.05). Non-parametric data were analyzed through Kruskal-Wallis test (p < 0.05). CT is control treatment, which corresponds to the medium without bacteria, and IT is inoculated treatment.