Figure 2.

H₂ reverses OVA sensitization and challenge-induced energy metabolic pathway switch. Mice in control (Con) and hydrogen-rich saline (HRS) groups were sham sensitized and challenged. Mice in ovalbumin (OVA) and OVA + HRS groups were sensitized and challenged with OVA. Mice in HRS or HRS + OVA group was administered HRS (6 ml/ kg /day, i.p.). At 24 hours after final OVA challenge, lungs were collected. Lung tissue levels of lactate, ATP, HK, PFK and CS activities, and lung mitochondrial OXPHOS complex I and III enzyme activities were measured. Means ± SEM of 5 mice per group. *p < 0.05, compared to control group. ^#p < 0.05, compared to OVA group (one-way ANOVA). (A,B) Lung lactate and ATP production. (C,D) Lung HK and PFK activities. (E,F) Lung mitochondrial OXPHOS complex I and III activities. (G) Lung CS activity.