Fig. 4.

E102 mutations abrogated drug response in σ1R oligomerization. (A-B) Representative western blots from five experiments are shown to visualize σ1R (red) and GAPDH (green) bands after drug treatment for E102Q (A) and E102A (B) constructs expressed in Δσ1R HEK293T cells. (C-D) Drug-induced changes in σ1R homomer BRET ratios are shown for the E102Q (C) and E102A (D) constructs expressed in Δσ1R HEK293T cells. None of (+)-pentazocine (red), PD144418 (cyan), or haloperidol (blue) induced significant changes in either of the mutants. Data represent mean ± S.E.M. (n = 5 or more). (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)