Figure 3.

Overexpressed lncRNA H19 Competitively Binding to miR-152-3p Affects Fibroblast Proliferation, Migration, and Apoptosis

(A) Binding sites between lncRNA H19 and miR-152-3p. (B) Subcellular localization of lncRNA H19 detected by FISH (400×). (C) Luciferase activity of H19-WT and H19-Mut detected by dual-luciferase reporter gene assay. (D) Relative enrichment of lncRNA H19 detected by RNA pull-down. (E) Relative enrichment of Ago2 by lncRNA H19 and miR-152-3p detected by RIP assay. (F) lncRNA H19 and miR-152-3p expression and mRNA expression of PTEN, determined by qRT-PCR. (G) Fibroblast proliferation detected by EdU assay (200×). (H) Fibroblast migration detected by Transwell assay (200×). (I) Fibroblast apoptosis detected by TUNEL assay (200×). **p < 0.01 versus the oe-NC group (fibroblasts treated with oe-NC); ##p < 0.01 versus the sh-NC group (fibroblasts treated with sh-NC). Measurement data were expressed as mean ± SD. Comparison between two groups was conducted using independent sample t test. One-way ANOVA was used for data comparison among multiple groups, followed by Tukey’s post hoc test. The experiment was repeated independently three times.