Figure 5.

MSCs Regulate the Expression of miR-152-3p and PTEN in Fibroblasts through MSC-exo Carrying lncRNA H19

(A) The uptake of MSC-exo by fibroblasts observed under an inverted microscope (400×). (B) Expression of lncRNA H19 in MSCs and exosomes, determined by qRT-PCR. (C) Secretion of exosomes in medium with exosome inhibitor added, observed under TEM (scale bar, 200 nm). (D) Expression of lncRNA H19, miR-152-3p, and PTEN, determined by qRT-PCR. (E) Protein bond diagram of PTEN determined by western blot analysis. (F) Relative protein expression of PTEN normalized to GAPDH, determined by western blot analysis. **p < 0.01 versus the control group (fibroblasts without any treatment); ##p < 0.01 versus the MSC oe-NC group (MSCs treated with oe-NC); &&p < 0.01 versus the MSC sh-NC group (MSCs treated with sh-NC). Measurement data were expressed as mean ± SD. One-way ANOVA was used for data comparison among multiple groups, followed by Tukey’s post hoc test. The experiment was repeated independently three times.