Figure 6.

MSC-exo Carrying lncRNA H19 Regulates Fibroblast Proliferation, Migration, and Apoptosis via the PI3K/AKT Signaling Pathway

(A) Fibroblast proliferation detected by EdU assay (200×). (B) Fibroblast migration detected by Transwell assay (200×). (C) Fibroblast apoptosis detected by TUNEL assay (200×). (D) Protein bond diagram of PTEN, p85 PI3K, and AKT, as well as the extent of AKT phosphorylation determined by western blot analysis. (E) Relative protein expression of PTEN, p85 PI3K, and AKT, as well as the extent of AKT phosphorylation normalized to GAPDH, determined by western blot analysis. **p < 0.01 versus the control group (fibroblasts without any treatment); ##p < 0.01 versus the MSC-exo-oe-NC group (MSC-exo treated with oe-NC); &&p < 0.01 versus the MSC-exo-sh-NC (MSC-exo treated with sh-NC). Measurement data were expressed as mean ± SD. Comparison among multiple groups was conducted using one-way ANOVA, followed by Tukey’s post hoc test. The experiment was repeated independently three times.