Figure 7.

MSC-exo Carrying lncRNA H19 Promotes Wound Healing in Mice with DFU

(A) Representative images displaying the wound-healing process of mice with DFU. (B) Wound area of mice with DFU. (C) Edge tissues of wound identified by H&E staining (200×). (D) miR-152-3p expression and mRNA expression of PTEN in wound tissues, determined by qRT-PCR. (E) Protein bond diagram of PTEN, p85 PI3K, and AKT, as well as the extent of AKT phosphorylation determined by western blot analysis. (F) Relative protein expression of PTEN, p85 PI3K, and AKT, as well as the extent of AKT phosphorylation normalized to GAPDH, determined by western blot analysis. (G) Levels of inflammatory factors (IL-1β, TNF-α, and IL-10), determined by ELISA. (H and I) Relative protein expression of angiogenesis-related factors (VEGF, TNF-β1, α-SMA, and collagen I) normalized to GAPDH, determined by western blot analysis. (J) Cell apoptosis detected by TUNEL assay (200×). **p < 0.01 versus the control group (n = 12, DFU mice without any treatment). Measurement data were expressed as mean ± SD. Comparison among multiple groups was conducted using one-way ANOVA, followed by Tukey’s post hoc test. Repeated-measures ANOVA was applied for data comparison among multiple groups at different time points, followed by Tukey’s post hoc test (B).