Table 1.
Gene | Primer Sequences |
---|---|
lncRNA H19 | F: 5′-TACAACCACTGCACTACCTG-3′ |
R: 5′-TGGAATGCTTGAAGGCTGCT-3′ | |
miR-152-3p | F: 5′-ACACTCCAGCTGGGTCAGTGCATGACAG-3′ |
R: 5′-CTCAACTGGTGTCGTGGAGTCGGCAATTCAGTTGAGCCAAGTT-3′ | |
PTEN | F: 5′-TTTGAAGACCATAACCCACCAC-3′ |
R: 5′-ATTACACCAGTTCGTCCCTTTC-3′ | |
U6 | F: 5′-CTCGCTTCGGCAGCACA-3′ |
R: 5′-AACGCTTCACGAATTTGCGT-3′ | |
GAPDH | F: 5′-AATCCCATCACCATCTTCCA-3′ |
R: 5′-TGGACTCCACGACGTACTCA-3′ |
lncRNA, long noncoding RNA; miR, microRNA; PTEN, phosphatase and tensin homolog deleted on chromosome ten; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; F, forward; R, reverse.