Figure 1.

Wnt7a Prevents Myotube Atrophy

(A) Experimental schematic outlining induction of myotube atrophy and treatment with Wnt7a or IGF-1 using murine primary myoblasts. (B) Measurement of the maximal myotube diameter demonstrates that myotube atrophy caused by conditioned medium from C26 colon carcinoma cells is prevented by addition of Wnt7a or IGF-1. (C) Representative images of myotubes from control conditions, after induction of myotube atrophy by C26 cell conditioned medium with and without Wnt7a or IGF-1 treatment. Images show myosin heavy chain (in green) and DAPI (in blue). Scale bars, 50 μm. n = 3. Error bars represent SEM. *p < 0.05, **p < 0.01.