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. 2020 Jan 21;16:179–191. doi: 10.1016/j.omtm.2020.01.002

Figure 2.

Figure 2

In Vivo GFP Transgene Expression after Suprachoroidal, Subretinal, and Intravitreal Injections of AAV8 in Rhesus Macaques

(A–C) Scanning laser ophthalmoscopy (SLO) montages and (D–F) magnified views of the red-dashed boxes in (A)–(C) showing different patterns of GFP expression after suprachoroidal (A and D), subretinal (B and E), and intravitreal (C and F) AAV injections. (G–I) Spectral domain-optical coherence tomography (SD-OCT) scans across the green dashed lines in (D)–(F), with (J–L) magnified views of the blue-dashed boxes in (G)–(I), showing impact on external limiting membrane (ELM), photoreceptor inner segment/outer segment junction (IS/OS), and retinal pigment epithelium (RPE) after suprachoroidal (G and J), subretinal (H and K), and intravitreal (I and L) AAV injections. ELM and IS/OS disruptions were seen in areas of greatest GFP transduction (open arrowheads) compared with adjacent areas with less transduction (closed arrowheads). Scale bars, 1 mm (A–F), 0.2 mm (G–L). ELM, external limiting membrane; IS/OS, inner segment/outer segment junction; RPE, retinal pigment epithelium.