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. 2019 Dec 20;12(2):e10919. doi: 10.15252/emmm.201910919

Figure EV2. Antisera detect poly‐GA aggregates specifically.

Figure EV2

  1. Immunoblot of HEK293 cells transfected with the indicated poly‐GA‐expressing construct and GFP control using monoclonal anti‐GA clone 1A12 and antiserum from Ova‐(GA)10‐vaccinated mice. Calnexin is used as a loading control. A representative of three experiments is shown.
  2. Immunoblot of HEK293 cells transfected with the indicated DPR‐expressing construct and GFP control using monoclonal anti‐GA clone 1A12 and antiserum from Ova‐(GA)10‐vaccinated mice. Calnexin is used as a loading control. A representative of three experiments is shown.
  3. HEK293 cells were transfected with (GA)175‐GFP or GFP control and analyzed by immunofluorescence using a mouse monoclonal anti‐GA antibody or antisera from vaccinated mice. Scale bar indicates 40 μm. A representative of three experiments is shown.