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. 2019 Dec 27;2(1):100065. doi: 10.1016/j.jhepr.2019.100065

Fig. 5.

Fig. 5

Evaluation of cellular replication in primary human hepatocytes in chimeric FRG livers.

(A) Quantitation of BrdU-positive human hepatocytes in engrafted FRG mice following vector treatment. Eleven weeks post-engraftment, mice received BrdU injections twice daily following vector delivery and livers were harvested 1 week later for immunohistochemical analysis. The number of BrdU-positive (brown) hepatocyte nuclei within human GAPDH positive clusters were counted across liver sections from engrafted FRG mice (n = 5 animals; 10 images per mouse). Representative images from 2 mice (FRG 297 and FRG 305) are shown. Scale bar = 100 μm. (B) Ki-67 labelling was determined in human GAPDH positive clusters at 11 weeks post-engraftment (n = 2 animals; 4 images per mouse). Representative images from the 2 mice (FRG 22 and FRG 23) are shown. Scale bar = 50 μm. BrdU, bromodeoxyuridine; FRG, Fah-/-Rag2-/-Il2rg-/.