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. 2020 Jan 31;10:1775. doi: 10.3389/fpls.2019.01775

Figure 2.

Figure 2

MYB51 and MYB122 are necessary for 4OH-I3M and 4M-I3M biosynthesis in ETI. (A) qPCR analysis of indole glucosinolate (GSL) regulatory genes and WRKY33 in 9-day-old wild-type (WT) seedlings co-elicited with 20 μM dexamethasone (Dex) and Pseudomonas syringae avrRpm1 (Psta) for 12 h. Data represent mean ± SE of four replicates of 13–17 seedlings each. Expression values were normalized to that of the housekeeping gene EIF4A1. SG12, subgroup 12 (B) HPLC-DAD analysis of I3M, 4-hydroxyindol-3-ylmethyl glucosinolate (4OH-I3M), 4M-I3M, and 1M-I3M in 9-day-old seedlings elicited with Psta for 24 h. DW, dry weight. The pen2 mutant cannot hydrolyze indole GSLs and thus over-accumulates defense-induced 4OH-I3M and 4M-I3M (Bednarek et al., 2009; Clay et al., 2009). Data represent the mean ± SE of four replicates of 13–17 seedlings each. Different letters denote statistically significant differences (P < 0.05, one-factor ANOVA coupled to Tukey's test). Experiments were performed twice, producing similar results.