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. 2020 Jan 31;11:32. doi: 10.3389/fendo.2020.00032

Figure 7.

Figure 7

Effect of HBO on levels of GLUT4 in skeletal muscle, Akt, and AMPK phosphorylation. (A) GLUT4 expression levels detected by immunofluorescence in skeletal muscle (400×) and measured intensity per arbitrary units. Arrows point to GLUT4 immunoreactive cells. Blue is DAPI (HFD: n = 3; HFD+HBO: n = 3; T2DM: n = 4; T2DM+HBO: n = 4). (B) GLUT4 levels in skeletal muscle measuring by western blot. (C) Western blot of p-Akt/Akt level in skeletal muscle (n = 3 per group). (D) Western blot of p-AMPK/AMPK level in skeletal muscle (n = 3 per group). Scale bar = 200 μm. Data are presented as means ± SEM. *P < 0.05, **P < 0.01 (compared with T2DM group), #P < 0.05 (compared with HFD+HBO group), ∧P < 0.05 (compared with HFD group).