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. 2020 Jan 31;11:32. doi: 10.3389/fendo.2020.00032

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Copyright © 2020 Liu, Zhang, Yuan, Song, Zhang, Lin, Li, Sheng, Ma, Lv, Gao and Dong.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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(A) Morphology of BAT and cell number counting. BAT morphology and UCP1 protein analysis after HBO. Partial BATs were cut into 5-μm sections stained using hematoxylin-eosin staining (400×) and numbers of cell from the same unit areas were counted (HFD: n = 3; HFD+HBO: n = 4; T2DM: n = 3; T2DM+HBO: n = 4). (B) UCP1 protein expression levels in BAT were measured (HFD: n = 3; HFD+HBO: n = 3; T2DM: n = 4; T2DM+HBO: n = 4). Data are presented as means ± SEM. Normal baric oxygen (NBO). Scale bar = 200 μm. *P < 0.05, **P < 0.01 (compared with T2DM group), ^#P < 0.05 (compared with HFD group), ^∧P < 0.05 (compared with HFD+HBO group).