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. 2019 Dec 21;8(2):e999. doi: 10.1002/mgg3.999

Figure 4.

Figure 4

Novel circ‐CMPK1/miR‐302e/cyclin D1 ceRNA regulatory network functions in vivo and in vitro. (a) The wild‐type and mutant sequence of miR‐302e and cyclin D1 binding site. (b) Luciferase reporter assay for wild‐type and mutant cyclin D1 3′‐UTR luciferase vectors in A549 and H460 cells transfected with control or miR‐302e mimics. (c, d) qRT‐PCR (c) and western blot (d) assays for cyclin D1 expression in miR‐302e‐overexpressing A549 and H460 cells with or without circ‐CMPK1 overexpression. (e) qRT‐PCR analysis for cyclin D1 expression in adjacent normal and NSCLC tissues. (f) The correlation between circ‐CMPK1 and cyclin D1 expression in NSCLC tissues. (g) Cell cycle assays with PI staining in miR‐302e‐overexpressing A549 and H460 cells with or without circ‐CMPK1 or cyclin D1 overexpression. (h, i) The volume and weight of tumors in nude mice bearing miR‐302e‐overexpressing A549 cells with or without circ‐CMPK1 or cyclin D1 overexpression (n = 5 in each group). * p < .05, ** p < .01, *** p < .001